Study The Effect of Aqueous and Alcoholic Extracts of Some Spices on Some Pathodenic Bacteria in Different Media

Abstract

Abstract : The effect of aqueous and alcoholic extracts of ginger , rosemary and thyme on the growth of many species of analytical and pathogenic bacteria in solid and liquid media was studied. Inhibitory of bacterial growth percentage in solid media was calculated by using disk method and adding the both kinds of extracts with concentrations as 10,15and 20 µg disk. The Minimum Inhibitory Concentration (MIC) was measured for each studied individual species of bacteria, of which it was between 25- 400 µgml. Results indicate that the two extracts have different Inhibitory effect depending on the kind , extract concentration and bacterial species . Further, aqueous extract of rosemary have more inhibitory effect on bacterial species than other aqueous extracts . Isolates of Pseudomonas sp. and Escherichia coli have the most resistance against different extracts . Alcoholic extracts of the three spices have more inhibitory effect than the others . Many isolates of the later bacteria species have resisted the extracts at different concentrations. However , Pseudomonas sp. was the most resistance bacteria against the aqueous and alcoholic extracts in the three spices and with lower degree for E.coli and Salmonella sp. at the concentrations which used in this study

Keywords

Diacetyl, Bacteria