STUDY THE EFFECT OF PURIFIED CATECHINS OF GREEN TEA ON THE MOST IMPORTANT CAUSES OF BACTERIAL INFECTIONS OF THE URINARY TRACT AND SYNERGISTIC EFFECT

Abstract

Two hundred urine samples were collected from patients infected with acute and chronic urinary tract infection, 85 bacterial isolate were isolated from these samples and were diagnosed using biochemical tests and kit API test, most of them identified E.coli (23.5%) K.pneumoniae rate (17.6%). Isolates were selected E.coli and K.pneumoniae according to their isolation rate from urinary tract infection cases and their antibiotic resistance. The inhibition effect of the purified catechin from the green tea was studied when the isolates were grown in the following concentrations (25, 50,75, 100) mg/ml. The concentration of 100 mg/ml showed a high action against the two species. The combination effect of the purified catechin was estimated with both Amikacin and Gentamycin on the resistant isolates to these antibiotics (E.coli and K.pneumoniae) and has been compared to the minimum inhibitor concentration of the isolates before and after the confusion of antioxidants, as the effect of mixing Amikacin and Gentamycin with Catechin, it gave a synergic effect with Amikacin on two isolates, either Gentamycin gave synergic effect on K.pneumoniae.