Isolation and Identification of Phenolic Compounds from Elettaria cardamomum Seeds and Study of their Medicinal Activity Against Pathogenic Bacteria of Prostate Gland

Abstract

Phenolic compounds were isolated from Elettaria cardamomum seeds by using organic and inorganic solvents. The chemical and physical properties of phenols were studied such as qualitative analysis, thin layer chromatography (TLC), functional groups test and infra-red spectroscopy. TLC results indicated presence of three compounds, two of them were flavonoids and the other was tanninic compound by using colour developers. The medicinal activity of phenolic compounds was measured and determined against growth of two pathogenic bacterial strains of prostate gland are Staphylococcus aureus , positive towards Gram stain (NCTC 6571) and Escherichia coli, negative towards Gram stain (NCTC 5933). The concentration of (50 mg/ml) was recorded to be the most activity towards both types of the pathogenic bacteria with inhibition zone diameter equal to (25 mm and 15 mm) for positive and negative bacteria respectively. The minimal inhibitory concentration (MIC) was determined with value equal to (12.5 mg/ml) for both types of pathogenic bacteria. As a result, the phenolic compounds can be used as herbal drug substituent for treating diseases of prostate gland inflammatory instead of used antibiotics but this work demands further pharmaceutical and clinical studies.