Study the efficiency of the enzyme glucose Oazomez product from the local isolation of the bacterium Streptomyces sp. HM5 in converting glucose to fructose

Abstract

used for producing High Fructose Corn Syrup (HFCS)for a long time ago . the enzymaticmethod is better than chemical method for producing HFCS . There are some organisms suchas Streptomyces can produce this enzyme. The aim of this research is to study the efficiencyof glucose isomerase produced from local isolate of Streptomyces by determination of theconversion of glucose to fructose in three sources (Corn syrup; date syrup and standardglucose).Three samples were used as a substrate glucose corn syrup, date syrup and standardglucose solutions. Partial purified enzyme (glucose isomerase) produced from Streptomycessp. HM5 was also used. Total and reduced sugars were determined by spectrophotometricmethod, specific rotation and TLC technique. The optimizations of time, substrate andenzyme concentrations were also studied.The results indicates that the optimum time for conversion glucose to fructose was 35– 40 hours in three substrats . The optimum concentration of glucose corn syrup, standardglucose and data syrup were 30; 20 and 10% respectively. The optimum volum of enzyme is0.5 ml with specific activity equal to 10. 80 U/ ml.The applied study shows that can be convert glucose to fructose in glucose corn syrup byusing glucose isomerase produced from Sterptomyces sp. HM5 and the product can be use inmany foods industries because of its higher sweeteners with low cost.