EVALUATION OF PERMANENT STAIN USED FOR FIXED SMEARS OF SOME INTESTINAL PARASITES IN STAINING OF URINE SEDIMENT SMEAR

Abstract

Urine samples were collected from patients complaining of urinary tract disturbances. Twenty samples were selected depending on the presence of leucocytes, erythrocytes, epithelial cells, and bacterial cells, detected by direct microscopic examination of urine sediment smear. The selected urine sediments were subjected to staining technique, with two types of stains: supravital stain using Löefflerُs methylene blue as a control stain and permanent stain for fixed stool smears of some intestinal parasites (PSFSIP). PSFSIP is a new permanent stain, is efficient and has simple and rapid procedure. Three types of cells were selected in this study. The staind cells appeared as follow: Neutrophil appeared with segmented violet nucleus located in granulated moderate blue cytoplasm; normal erythrocyte appeared as a pink colored disc, and dysmorphic erythrocyte appeared as a pink crescent, both were surrounded by dark violet line, Epithelial cell appeared with pale blue cytoplasm and round or oval dark bluish violet nucleus, while bacterial cells appeared with dark blue color. This stain is simpler and efficient in staining of the color and morphology of the cellular constituents of the urine sediment smears were found to be stable and clear for entire one year.