Spectrophotometric Determination of Isoniazid Via Periodate oxidation In Pharmaceutical Tablets


The research demonstrates the development of spectrophotometric method for the determination of isoniazid using periodate oxidation in sodium bicarbonate media to produce iodate, excess periodate was masked by molybdate, then iodide was added to react with iodate in acidic media to produced triiodide, which absorbance mesured at 355 nm. The molar absorptivity is 1.564105 l.mol-1.cm-1 and determining range is 2-24μg/25ml. The accuracy (average recovery) is 101.3% and a precision (RSD) is less than 5%. This method has been applied successfully to the analysis of isoniazid in pharmaceutical tablets. The results obtained are in agreement with certified values and also with D-PNA method.