Synthesis and study effects of 3,4-Dihydro-4-(p-anisyl)-6-phenyl pyrimidine-2(1H)-one on growth and morphology of Leishmania tropica promastigotes in vitro

Abstract

Most of the drugs used in the treatment of leishmaniasis have side effects, there is a need for a safe and effective drugs, therefore, in the present study heterocyclic compound containing pyrimidine ring ( 3,4-Dihydro-4-(p-anisyl)-6-phenyl pyramidine-2-(1H)-one ) was synthesized, then tested for there antileishmanial activity against Leishmania tropica promastigotes in vitro. The effect of the compound on the growth, generation number and generation time of the parasite was determined using concentrations ranging from 1 to 10 µg/ml.The effect of the compound concentration was found to be important to the parasite growth as the more the concentration the less the growth, generation number and increased the generation time. At the log phase the IC50 and IC90 were 2.5 and 10 µg/ml respectively and caused morphological distortions.The toxicity of the synthesized compound was determined using Balb/c mice model. Dixon's up and down method ( 1980 ) was found to have an LD50 of 642.5 mg / Kg of body weight.