Inhibitory effect of local Honey on Bacteria in culture media and in laboratory animals


This study was carried out at the animal station agriculture and the food technology research center of Iraqi ministry of science & Technology. In a study to determine the inhibitory effect of honey in the local growth of some pathogenic bacteria in growth media (In vivo) and in laboratory animals (Invitro) was used four types of bacteria brought from the Central Health Laboratory - Baghdad, Escherichia coli , Streptococcus pneumoniae , Staphylococcus aureus , Streptococcus pyogenus and Pasteurella hemolytica, and their biochemical characteristics were re-examined.The study deals with studying the effect of local honey in the inhibition of the growth of these bacteria on environmental Nutrient Agar (Invitro) to measure the diameter of zone inhibition after incubator the culture media for a period of 24-48 hours, as for the study of the effect of honey in laboratory animals(mouse In vivo) 12 groups of local mice were used, in addition to four mice of each control group. Each group is given (cfu / ml 104 ) of specific bacteria once and then it is given Diluted honey of different concentrations (5%,10%,20%)with drinking water for one week to all groups except the control group.The study showed a different degree of inhibition of honey depending on the type of bacteria and whether the bacteria is negative or positive of the dye gram and the inhibitory effect of the honey in the nutrient broth increased according to the concentration of honey. The results in animals laboratory differed sometimes depending on the type of bacteria, this indicates that local honey is good in efficiency in reducing bacterial growth which might qualify for use as a treatment supporter with drugs and this is what must be expanded in future studies.