Preparation of a New Medium for the Isolation of Viridans Streptococci

Abstract

Three types of media were prepared from the crude extract of pumpkin fruit, the first medium was prepared from the crude extract alone (Medium A1), the second was made by the addition of peptone to the crude (Medium A2) and the third was made by the addition of trypan blue, crystal violet and potassium tellurite (Medium A3) as selective agents for the isolation of S. mutans, these three types of media was examined for the growth and isolation of the Viridans Streptococci and S. mutans, all of them were good and there was no need to the addition of peptone (M. A1), the growth of S. Salivarius and S. mutans was differentiated from other Viridans Streptococci from saliva sample, by their large colonies, the A3 was selective for S. mutans by gummy, large and smooth colonies.