Some Biological Effects of Pimpinella anisum L. Seed Extract on Culex pipiens molestus F.


Different concentrations of seed extract of anise Pimpinella anisum L. were tested against 2nd instar larvae of mosquito Culex pipiens molestus F. One hundred percent mortality was observed at 10 µg/ml and the LC50 was 7 µg/ml after 48h following exposure.Results showed that seed extract was effective up to 30 days of storage. The seed extract exhibited growth-inhibiting activity. Various morphogenetic aberrations was found in the mosquito after treatment of 2nd instar larvae. Most of the treated larvae died in the larval exuvium. Unmelanized and melanized pupae were also noted and the adults were unable to free their appendages.