The antimicrobial effect of water extraction of Salvadora persica (Miswak) as a root canal irrigant


The aim of this study was to evaluate the antimicrobialeffect of 10% water extraction of Salvadora persica (Miswak)when used clinically as an endodontic irrigant.Twenty four uniradicular teeth with necrotic pulps werechosen. The patients were divided randomly into 2 groups:Experimental group, in which water extract of Salvadorapersica (10%) was used as a root canal irrigant; and controlgroup, in which distilled water was used as a root canal irrigant.Bacteriological samples were obtained from the canal atthe step of working length determination (before the canalwas subjected to instrumentation and irrigation procedures),and at the end of the biomechanical instrumentation proceduresby using a sterile K–file. The file was separated from thehandle using a sterile wire cutter, and the severed portion wasplaced in a sterile screw–capped vial containing 5 ml of thioglycollatebroth as a transport media. A 0.1 ml of thioglycollatebroth was inoculated on each of two brain–heart infusionagar plates: One plate was incubated under aerobic conditions,and the other was incubated under anaerobic conditionsusing anaerobic jar and gas pack anaerobic system. Both plateswere incubated at 37 ºC for 24 hours; then, the number ofbacterial colonies was counted.The results revealed that 10% water extraction of Salvadorapersica is an effective antimicrobial agent when utilizedclinically as an irrigant in the endodontic treatment of teethwith necrotic pulps.