Abstract

Spironolactone SPL stock solution was studied against two cancer cell lines Hep-2, and AMN-3. These cell lines wereexposed to different concentrations of SPL ranged from 0.0019 to 1000 μg/ml for 24, 48 and 72 hours durations. Theoptical density was measured under wavelength 492 nm with micro-ELISA reader after their staining with crystal violetdye.The results revealed a clear cytotoxic activity of pure SPL with high significances in a two cancer cell lines during thethree exposure time, suggesting that the cytotoxic effect of pure spironolactone is a dose and time dependant. The bestcytotoxic activity was on AMN-3 and Hep-2 cell lines at the high concentrations 1000, 500, 250, 125μg/ml.The results also suggest that AMN-3 cell line is the most sensitive cancer cell line to pure SPL than Hep-2 cell lineafter 24 hr. of exposure to drug.

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The article was added to IASJ on 2012-06-21

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