STUDY OF ANTIMICROBIAL ACTIVITY OF SALIX

Abstract

ABSTRACTAntimicrobial activity of cold aquous and ethanol extracts with six concentrationsof each one (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) mg/ml of Salix acmophylla leaves wereestimated against four types of pathogenic bacteria (E. coli, S. aureus, Klebsiellaspp., St. pyogenes) after incubation at (37)oC for (24)hr. by using well diffuionassay method. Antimicrobial activity of both extracts was compared with that for anumber of antibiotics include Ampicilin, Cephalexin, Erythromycin, Amoxicilin,Gentamicin and Tetracyclin, by using antibiograme test. Both extracts of Salixacmophylla showed clear antimicrobial activity against pathogenic bacteria. Thisactivity was enhanced with the increasing of extracts concentrations. The 0.6%concentration of both extracts gave highest activity against E. coli, S. aureus andSt. pyogenes with no antimicrobial activity against Klebsiella spp. E. coli was theleast testing bacteria affective by the concentrations of both extracts. While S.aureus was the most effective one by the concentrations of aquous extract and St.pyogenes was the most affective one by the concentrations of ethanolic extract.Antimicrobial activity of cold aquous and ethanolic extracts of Salix acmophyllawas better than that to Gentamicin against St. pyogenes and S. aureus, while theinhibition effect of Tetracyclin was better than both extracts concentrationsagainst S. aureus and E. coli. The inhibition effects of Gentamicin andErythromycin and both latest two concentrations of both extracts against E. coliwere closely related. Klebsiella spp. was sensitive to Gentamicin only. A resist ofpathogenic bacteria to Ampicillin, Amoxicilin and Cephalexin was observed.