STUDY THE EFFECT OF LACTOBACILLUS AS

Abstract

ABSTRACTThis study included to investigate the types of Lactobacilli which found in the milkof 35 nursing mothers, and study the effects of these normal friendly bacteria onCandida albicans. Eleven species of the genus Lactobacillus were isolated anddistributed as ; thirteen isolate belong to L. plantarum (26 %) , (12) isolate to L.gasseri (24 %) , while L. acidophilus represent (6) isolates (12%) , L. fermentum(5)isolates (10%) , L. casei (3) isolates (6 %) , beside 2 (4 %) isolates for each of L.lactis , L. rafinotactis , L. paracasei , L. rhamnosus , and L. crispatis and only oneisolate belongs to L. salivaris (2%) . Candida albicans was diagnosed in 25 samplesof saliva from infants affected by thrush. The susptibility of C. albicans was testedagainst five antifungal drugs The results revealed that the isolates were sensitiveto Miconazole , Ketoconazole and Clotrimazol , the zone of inhibition reached to11, 10 and 10 mm. respectively ; on the contrary, the isolates were resistance tothe Nystatin and Grisofulvin . The effect of Lactobacillus spp. filtrate wereexamined against C. albicans and the results were clarified that there wasinhibition activity of L. acidophilus and L. fermentum against it, with zone ofinhibition reached to (28), (15) mm. respectively. When the effect of Lactobacillusspp. extract on C. albicans DNA was measured , by spectrophotometer at differentwave lengths ( 260, 280, 361 nm ) respectively they showed increase of theabsorbance with increasing of the amount of extract , which would mean that theextract cause distraction to the DNA of the C. albicans .