STUDY THE ANTIMICROBIAL ACTIVITY OF PROANTHOCYANIDIN (POCS) FROM GRAPE SEEDS

Abstract

ABSTRACTProanthocyanidin (POCs) was extracted from grape seeds by liquid-liquidchromatography and identified via high performance liquid chromatography(HPLC).The antimicrobial activity of POCs was determined against nine of grampositive and gram negative bacterial strains and Candida albicans by agar welldiffusion method and two strains of dermatophyte fungi Trichophyton interdigitaland Trichophton menagraphytes. It was found that POCs has inhibition effectagainst Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilitis whichgave inhibition zone (16, 14.5, 11) mm/ml respectively at the concentration 100mg/ml While there was low no inhibition effect against gram negative bacteria andCandida albicans, On the other hand the inhibition percentage againstdermatophyte fungi T. interdigital and T.mentagraphytes increased with increasingthe concentration of POCs which reached for 64.7%and 44.4%respectively