Effect of plant growth regulators on adventitious buds formation from date palm callus (Phoenix dactylifera L.)cv.Barhee in vitro


The present work was conducted to study the effect of plant growth regulators on the formation of adventitious buds on the callus of the date palm cv.Barhee .A primary callus was used ,which was obtained by culturing the apical and axillary buds of Barhee offshoots .The explants were placed on solid basal (MS)medium with activated charcoal and supplemented with the auxin(NAA) at 30mg. litre-1and the cytokinin (2ip)at 3mg litre-1.The nutrient media were determined, for each proliferation stage, starting with the establishment of culture ,till the stage of plantlet regeneration from the adventitious buds. The effect of various combinations of auxins and cytokinins were tested .The Results can be summarized as follows:-1.Results emphasized the vital role of plant growth regulators in the induction of adventitious buds formation the callus. The combination NAA at 1 mg litre-1 and the cytokinin (2ip and BA at 5 mg litre -1)caused the formation of adventitious buds within 165.6 day, and buds number was 13.6. The same combination also gave the highest number of proliferated adventitious buds (15.3 buds). 2.Results showed that it،s at paramount importance for the presence of elongation of shoots derived from adventitious buds, which required the addition of GA3 to the culture media at low concentrations GA3 at a concentration of 5 mg litre-1 gave vigorous shoots ,with an average height of 5.9cm . The addition of adenine sulphate at concentration of 70 mg. litre-1 also produced good and vigorous shoots ,in addition to the use of sucrose at 50g. litre-1.3.Results showed, the importance of the auxin NAA, in the stimulation of rooting of shoots that produced from adventitious buds, with the height, number of roots (4.8) and length were obtained with concentration of 0.5 mg litre-1 NAA, as compared with the same concentration of IBA.