Serum Resistin and Anthropometric parameter in lean and Overweight Insulin –Dependent Diabetic

Abstract

Background: Type 1 diabetes mellitus is a multifactorial syndrome due to a body's inability to synthesis insulin. In patients with type 1 DM, the beta cells are attacked by the immune system (specifically by macrophages and T lymphocytes) and killed, so insulin can no longer be synthesised. Resistin is a newly identified adipocyte secreted hormone belonging to a cysteine-rich protein family. Human resistin gene is expressed in pancreatic islets , pre- adipocytes and bone marrow. It is of relevance for inflammation processes as well as for lipid metabolism. The body mass index BMI a measured of relative weight, adjusted for height. Resistin serum levels were found to be related to body mass index (BMI) in human subjects. Waist circumference is minimally related to height, so correction for height (as in waist to height ratio) dose not improve its relation with intra- abdominal fat or ill healthObjective: The aim is to determin the role of Resistin in lean and overweight .Subjects and methods: sixty diabetic children ( 33 males and 27 females), aged (1.3-13) years (mean± SD) (5.6±2.8) years , were enrolled in this study. All had T1DM with no serious long- term complications and C-reactive protein for all patients was negative. All patients used short- and intermediate-acting subcutaneous insulin injections (two daily injections) and no other medication. Duration of disease varied from ( 0.13- 84) months . A matching group of thirty healthy volunteer children with age range of (1.3-13 ) years, (18 females ,12 males) were included as a control without any family history of diabetes. Serum Rresistin was measured by( enzyme linked immunosorbant assay , ELISA) . Anthropometric determined are measured as BMI and WHR. BMI (weight in kg divided by the square of the height in meters), Waist and hip circumferences were measured to determine waist-hip ratio (WHR) following standardized procedures .Results: Resistin values showed no significant difference when comparing both groups (patients and controls). A significant decrease in the level of fasting serum Resistin was found in overweight patients when compared to control, while no such significance was shown for serum Resistin in lean patients when compared to controls. However a significant correlation was found when comparing serum Resistin and BMI in controls but the correlation was not significant in patients.Conclusion : Resistin levels was found to be highly associated with obesity in healthy controls with a significant elevation in overweight when compared to lean sunjects. However the opposite was found in patients under treatment with insulin. The latter was found to have a depleting effect on Resistin gene expression.