Enzymatic Markers of Salivary Cell Injury in Saliva of Type 1 Diabetic Children


Abstract:Salivary components may suffer variations that can be detected by chemical determinations. The salivary glands may be additional target of the immunological attack mainly directed against pancreatic beta-cells resulting in type1daibetic children. Changes in enzymatic activity reflect metabolic changes in diabetic children and inflammation. Aim of the study: The work is devoted to prove the hypothesis that changes in glucose concentration, aminotransferases, lactate dehydrogenase can be used as indices of cellular injury in the whole saliva of diabetic children, and to determine the physical and biochemical characteristics of saliva of a group of diabetic children compared to a control group in relation to oral health indices.Subjects and methods: Twenty six diabetes children (5-19 years) and twenty control children (5-11 years) were included in this study. Glucose, aspartate and alanine aminotransferase (AST, ALT), and lactate dehydrogenase (LDH) were determined by enzymatic methods. Results: Obtained results had shown statistically significant increases of saliva glucose level and increase in the activity of AST, ALT and LDH in saliva from diabetic patients and control group. The results also demonstrated that acidic pH diminishes salivary flow rate and excess foam is usually present in saliva of diabetic children.Conclusion: These differences were confirmed by the discrimination test. Diabetic children have higher DFS (decayed filled surfaces) compared to those of control children. Some salivary components in addition to the diminished flow rate could be involved in the characterization of the oral health state of diabetic children.