Buffy Coat smear and culture: An early diagnostic procedure in bacteremic and septicemia Pediatric Patients

Abstract

Blood culture is the single most important procedure to detect systemic infection due to bacteria, it provides valuable information for management of febrile. In this study trying to use Buffy coat smear methods and culture by using unclotted peripheral blood in haematocrite capillary tube, which may yield important early diagnosis procedure in bacterimic and septicemic baby especially in critically ill and low birth weight infant. Among sample of Iraqi childhood a study done on 280 babies patients clinically diagnosed to had bacterimia and septicemia. the frequency among male babies was higher than that of female, the ratio 1.5: 1 , the occurrence was more noticeable under one year age group represent 58.9% and decline among other age group in which positive culture showed in 75%(210/280) were male represent 60% of the isolate while female represent 40%. As a conclusion from this study Eshericha coli considered the main organism isolated represent 16.7% (35/210), Pseudomonase spp represent 15.2%(32/210), Streptococci spp 14.8%(18/210), Klebsiella spp 13.3%928/210), Eterobacter spp 11.4%(25/210), Salmonella 8.6%(18/210), finally staph-albus isolated from 6.2% immuncompromised patients. Associated disease status as arise factor for bacterimia and septicemia including meningitis 18.6%, urinary tract infection 17.6% , chest infection 13.4%, intestinal obstruction 12.4% , jaundice 11.9%, pneumonia 9%. Kal-azar and galactecemia represent 5.2% respectively.