New approach development for determination of chlorpromazine HCl in pure and pharmaceutical forms using homemade wave length selector flow injection photometer


A simple , accurate and fast method for determination of chlorpromazine HCl in pure and drug formulations by continuous flow injection spectrophotometric was developed. The method was based on the oxidation of the drug with sodium persulphate to pinkish-red species and the same species was determined using homemade Ayah3Sx3-3D-solar flow injection photometer. Optimum conditions were obtained using the high intensity green light Emitted Diod as source . A 180µl was taken as a reasonable sample volume for the determination of drug in pure and pharmaceutical formulation. The optimum conditions reached were 1ml/min flow rate (10mmole.L-1) for sodium persulphate and 15seconds allowed time for injection. The linear dynamic range for the instrument response versus chlorpromazine HCl concentration was 0.05-0.6 mmole.L-1 while the L.O.D was 64.8nM/sample from the stepwise dilution for the minimum concentration of lowest concentration in the linear dynamic range of the calibration graph . The correlation coefficient (r) was 0.9959 while the percentage linearity (%r2) was 99.19% . R.S.D% for the repeatability (n=5) was < 1.5% for the determination of chlorpromazine HCl with concentrations of 0.15 and 0.18 mmole.L-1 . The method was applied successfully for the determination of chlorpromazine HCl in pharmaceutical preparation . Using paired t-test it was shown that there were no significant difference between the proposed method and official method and on that basis the new method can be accepted as an alternative analytical method.