SPECTROPHOTOMETRIC DETERMINATION OF HUMAN SERUM ALBUMIN BY A HOME-MADE SEMI-AUTOMATED

Abstract

The bromcresol green method was applied for spectrophotometric determination of albumin by using a home-made semi-automated flow injection in some pharmaceutical preparation with recoveries of 98.5-102 % and 54 serum samples of male and female patients in the range 32.48-51.25 g/L.The method is convenient, simple and sensitive with detection limit (2x noise) 0.625 g/L. The r.s.d % is 0.74 % for 8 measurements of 40 g/L albumin.