SPECTROPHOTOMETRIC DETERMINATIONOF TETRACYCLINE IN SOME PHARMACEUTICALPREPARATIONS

Abstract

This paper briefly describes a simple spectrophotometric method for the determination of tetracycline in pharmaceutical preparation . The method is based on measurement of the absorbance of tetracycline- cerium(IV)complex at 430 nm in 1M sulphuric acid solution. Calibration graph for tetracycline was obtained over the range of 50-350 µg.ml- 1 The detection limit (2x noise) is 0.0025 µg.ml- 1 and with R.S.D for 5 replicate 0.03% .This method allowed the determination of tetracycline in pharmaceutical preparations with a satisfactory accuracy when comparing it with official BP method.