Measurement of Total Antioxidant Status (TAO) and Superoxide dismutase (SOD), Catalase (CAT) Enzymes in Petrol Station workers

Abstract

Background: Total antioxidant status, superoxide dismutase (SOD) and Catalase (CAT) are antioxidant defensive enzymes that catalyze the reduction of reactive oxygen species (ROS) to non harmful substance. Aim: The study focuses on the serum super oxide dismutase enzyme level, CAT level and total antioxidant status in petrol station workers to detect any imbalance between aggressive and defensive factors.Materials & Methods: the study included 40 workers and 20 healthy subjects in a comparative analysis. (TAO), serum (SOD) and (CAT) levels were measured for study subjects. Results: Statistical analysis of serum antioxidant enzymes level and antioxidant status revealed a significant increase in SOD, CAT of worker groups (p.≥0.05), and (P.≥0.01) respectively. The TAO was significantly increased (p.≥0.001). The increased of serum SOD, CAT levels and the TAO can be explained on the basis of alteration on enzymes activity, which may lead to disturbance in homeostasis of antioxidant/oxidant balance. Conclusions: TAO, Catalase and Superoxide dismutase enzymes can used as a biomarker of enzymatic alteration in different diseases.Key wards: Total Antioxidant Status, Super Oxide dismutase, Catalase, Petrol Station Workers.