Oxidative Stress and the Antioxidant Mechanisms in a Sample of Iraqi Patients with Polycystic Ovary Syndrome (POS)


Objective: The aim of this study was to investigate the levels of non enzymatic antioxidants glutathione (GSH), vitamin C and vitamin E and enzymatic antioxidants glutathione peroxidase (GPx), glutathione reductase (GRx), superoxide dismutase (SOD), catalase (CAT), glutathione s- transferase (GST) in patients with polycystic ovary syndrome.Patients & Methods: Fifty women with PCOS and fifty healthy, age and body mass index (BMI) matched controls were evaluated in this controlled clinical study. Erythrocyte GSH, plasma vitamin C, vitamin E, and activities of antioxidant enzyme SOD, GPx, GRx, catalase and GST were estimated. Oxidant status was evaluated by determination of erythrocyte malondialdehyde (MDA) concentration.Results: The women with PCOS had significant increase in the erythrocyte MDA levels compared to controls (P < 0.05). The activities of erythrocyte antioxidant enzymes SOD, GPx, GRX and plasma GST were significantly increased in PCOS patients compared to healthy subjects, while levels of erythrocyte GSH, Vitamin C, vitamin E and catalase activity were significantly decreased in patients with PCOS compared to healthy subjects.Conclusion: An increase in oxidant status was found in women with PCOS.The results of this study suggest higher oxygen free radical production, evidenced by increased a marker of lipid peroxidation (MDA) and decreased GSH, vitamin C & E, and catalase activity, support to the oxidative stress in PCOS patients. The increased activities of antioxidant enzymes may be a complementary regulation in response to increased oxidative stress.Keyword: antioxidant / PCOS / MDA / enzymatic and non enzymatic antioxidant