Effect of Clove Oil on Adhesion of Staphylococcus aureus to Buccal Cavity Epithelial Cells In vitro

Abstract

Background: Adhesion is an important starting event in the pathogenesis of bacterial infection. The microorganisms must first adhere to host tissue in order to multiply and create a colony or colonies before symptoms allow the disease process to be detected. Clove oil has been reported to possess interesting antimicrobial effects on various microorganisms.Objective: The objective of this study was to assess whether clove oil interferes with the adhesion of S. aureus to buccal epithelial cell. Methods: Adults apparently healthy volunteers (forty) were included in this study. Adhesion assay has been carried out in this study by mixing S. aureus bacteria with the buccal epithelial cells and incubated with & without clove oil. Antibacterial activity of clove oil was determined by using disc diffusion assay as well as tube dilution assay.Results: The percentage of epithelial cells with adhered bacteria decreased from 97.78% to 91.1% with a P value > 0.05, also the mean number of adhered bacteria per epithelial cells was decreased from 116.76 to 12.55 after oil treatment with a P value < 0.05. The antibacterial test revealed that the minimal inhibitory concentration for S. aureus was 1/256 (0.391(v/v)) of the original clove oil preparation Conclusion: Clove oil which has antibacterial activity to Staphylococcus aureus, has an inhibitory effect on adhesion of Sta-phylococcus aureus to buccal epithelial cells Key words: Clove oil, Staphylococcus aureus, adhesion, buccal epithelial cells. Control group were enrolled in the present