Isolation and Identification of Some Bacteria and Candida albicans Causing Vaginitis


Background: The vagina normally contains harmless bacteria which help to clear out unwanted germs. If these usually harmless bacteria increase in numbers and change the concentration of bacteria in the vagina a condition known as bacterial vaginitis can result. Objective: The aim of this study was to isolate and identify microorganisms that may cause vaginal infection and then testing their antimicrobial sensitivity. Methods: Vaginal swabs from 210 women (pregnant and non pregnant women) that attending Al-Yermouk Teaching Hospital in Baghdad from September 2007 to December 2009. Swabs identified and tested for antimicrobial resistance by well known established microbiological methods.Results: Out of 210 isolates, 186 were positive. These are 82(44%) Gram positive isolates and 66 (35.43%) Gram negative isolates, in addition 38 (20.5%) Candida albicans. The number of isolates was 50(26.88%) Gram positive in pregnant women and 32(17.20%) in non pregnant, while 42 (22.58%) were Gram negative in pregnant women and 24(12.9%) in non pregnant. The highest isolate is Escherichia coli 28(15%) and it was highly sensitive to cefotaxime (90%), while it was resistant to chloromphenical (20%). On the other side Staphylococcus aureus which was rack the second among the isolates 25 (13.44%) was mostly sensitive to cloxacillin (85%). The majority of Gram negative isolates were resistant to the co-trimoxazale except Klebsiella.Conclusion: It can be concluded that the Gram positive bacteria are more predominant than Gram negative bacteria in vaginal infections. The highest prevalence isolate is Escherichia coli followed by Staphylococcus aureus and Candida albicans. Also this study indicated that vaginitis is more common in pregnant women (in first and second trimesters) than in non pregnant one as a result of many factors such as hormonal factors and low socioeconomic status.Keywords: Vaginal infections, bacteria and Candida spp., antibiotics sensitivity.