Alleviation of cisplatin-induced nephrotoxicity in rats by aqueous extract of Salvia officinalis leaves

Abstract

Objectives: To evaluate the protective effects of the aqueous extract of Salvia officinalis leaves against nephrotoxicity induced by cisplatin in rats. Methods: Albino rats were divided into three groups, each group consist of six rats. Group I treated with vehicle (distilled water) was kept as a control. Group II injected with a single dose of cisplatin (12 mg/kg body weight; i.p.). Rats in Groups III were received a single daily dose of aqueous extract of S. officinalis 100 mg/kg (P.O.), for 7 days. On the seventh day, cisplatin (12 mg/kg body weight; i.p. was administered half an hour after the last dose of the plant extract. The rats in all groups were sacrificed 72 h after treatment. Renal injury was assessed using serum biochemical markers (creatinine and urea).Malondialdehyde (MDA) concentration was measured as a marker of lipid peroxidation.The renoprotecive activity of S. officinalis was supported by histo- pathological studies of the kidney.Results: Aqueous extract of S officinalis leaves significantly protected rat kidneys from cisplatin-induced histopathological changes. This extract also normalized cispaltininduced increases in serum creatinine and blood urea. In vitro studies revealed that the S officinalis leaf extract possessed significant oxidative radical scavenging activities.Conclusion: Both biochemical findings and histopathological evidence showed the renoprotective potential of aqueous extract of S. officinalis leaves against cisplatin-induced oxidative stress and renal dysfunction in rats.