Improved selection medium and technique for isolation of Azospirillum irakense


Abstract:An improved selection medium and technique for Isolation of Azospirillumirakense was developed . This technique is based on the utilization of free-nitrogensemi-solid medium supplement with disaccharide, lactulose, as a sole source ofcarbon and energy instead of malate with a pH value of 7.4 .This selected mediumwas found to encourage the growth of A. irakense alone and to prevent the otherspecies of Azospirillum.The improved medium was superior than the other available media .Thetechnique was simply applied to detect A.irakense, showed a high increase innumbers of A.irakense isolates obtaining from the soil rhizosphere and roots, anddecreased the time cost.