Isolation and screening of lactic acid producing Lactobacilli

Abstract

Twenty bacterial isolates belonged to the genus of Lactobacillus were obtained from different sources included raw milk , pickles , rural dairy, whey and yoghurt . The above isolates in addition to four isolates belonged to the same genus were subjected to a screening program to test their abilities for lactic acid production using different incubation conditions . Results showed that L. acidophilus and Lactobacillus sp. (25) were the higher producer isolates for lactic acid .The best medium for production of lactic acid from L. acidophilus and Lactobacillus sp. (25) was date juice in comparision with MRS liquid medium ,the lactic acid amounts were (44.02 and 64.8) gm/L by using date juice as production medium, while they were (36.12 and 62.076) gm/L by using MRS medium for both used isolates , respectively.