Genetic Study on Ampicillin Resistant Streptococcus pneumoniae Isolated from Suppurative Otitis Media

Abstract

A total of 217 ear swabs were collected from patients infected with suppurative otitis media through their visit to ENT unit of Al-Zahrawi Hospital in Mosul city. Isolation and identification of microorganism from all specimens were done, the role of Streptococcus pneumoniae as a causative agent of the disease is shown; finally we examined plasmid existence in Streptococcus pneumoniae and it's role in ampicillin resistance. The result showed that this bacterium is one of the main causative agents of otitis media.Plasmids have been prepared from Streptococcus pneumoniae and calculated their quality; the results also showed that the gene responsible for this bacterium resistance to ampicillin is located on chromosomal DNA, and plasmids have no role in this reistsance. Finally these genes were transferred from resistant strain to a sensitive one by transformation process