Isolation and Diagnosis of Lipoteichoic acid Isolated from some Gram Positive Bacteria

Abstract

In this study, Locally isolated Staphylococcus aureus and Streptococcus viridans are used as a source of Lipoteichoicacid (LTA) which was isolated by Affinity Chromatography.Some diagnostic tests were done to detect the presence and purity of LTA after measuring the absorbance of the samples using 200-400 nm wavelength. LTA samples were diagnosed qualitatively by thin layer chromatography (TLC) by measuring the rate of flow which reached 0.68, then were diagnosed by high performance liquid chromatography (HPLC) by calculating the retention time, which reached 2.03 minutes for S. aureus - LTA and 1.96 minutes for St. viridans- LTA .