Motility Types for Pseudomonas aeruginosa Isolated from Respiratory Tract Infections


Motility types of Pseudomonas aeruginosa (P.aeruginosa) have been studied and the detection on twitching motility was done by using stabbing with 1% agar to Luria-burtani (LB) broth medium. P. aeruginosa isolates revealed the ability to twitching motility which was characterized by extension of growth between agar and Petri dish, In addition to various degree of swarming after induction by using different amino acids and different agar concentrations lower than 0.7%. Cell morphology was examined from the center and margins of swarmed cells, cells from center seemed normal in morphology while the marginal cells appear elongated. Also swimming motility was observed by the wet mount preparation. The elongated cells were showed from LB broth by using transmission electron microscope. The bacterium succeeded in swimming motility in minimal media with 3% agar