Purification , characterization of chitinase produced from Bacillus subtilis and inhibitory activity on some fungi

Abstract

Bacillus subtilis with highest production of chitinase was selected among other Bacillus sp. Chitinase was extracted from culture media contain shrimp and fish-shell powder as the major carbon. Enzyme was purified by anion exchanger (DEAE) and Sephadex G-50 Gel filtration with yield and Purification fold, 33.18% and 11.01 respectively. Optimum PH for activity and stability was 6,5 and 5.5-8 respectively, The purified enzyme had Mw. 37 Kd. Inhibitory activity was 100% against some molds after incubation for 2h with sterilized chitinase solution (Unit).