Antibacterial activity of Silver Nanoparticles Using Stem bark of Juglans regia against, Streptococcus mutans , Streptococcus. sanguis and ,Porphyromonas gingivalis

Abstract

Dental careis & periodontal disease ,the most widespread diseases affectingmankind ,involve the adherence of bacteria & development of biofilms on both thenatural & restored tooth surface .within this context, a biofilm can be Classed as anaggregate of micro-organisms in which cell adhere to each other & to a surface.Nanotherapeutics offers the possibilitiy to control the formation of these & other oralbiofilms through the use of nanoparticles with biocidal ,anti-adhesive & deliverycapabilities.In this study, we investigated the antimicrobial activity of silver nanoparticles(Ag-NPs) using medicinal plant extracts (Stem bark of Juglans regia ) against,Streptococcus mutans, Streptococcus. Sanguis, and Porphyromonas gingivalis.Extracellular biosynthesis of silver nanoparticles was carried out by usingmedicinal plant extracts for the reduction of aqueous silver ions in short period. Thesilver nanoparticles formation was confirmed by the color change of plant extracts(SNPs) and further confirmed with the help of UV-Vis spectroscopy. Antimicrobialeffect of Ag-NPs against, Strep.mutans, Strep. sanguis , and Porph. gingivalis wasinvestigated by using disc diffusion method.The growth of Gram-positive Strep.mutans, Strept. sanguis (the causative agentsof careis) , and Porph. Gingivalis (the causative agents of Periodontitis) wereinhibited by Ag-NPs. The highest antimicrobial activity was observed against Strep.mutans (15.6mm SD± 1.6), Strept. sanguis (13.3mm SD±1.4) and the least wasnoticed against Porph. gingivalis (12mm SD± 1.0).It is confirmed that silver nanoparticles are capable of rendering high antibacterialefficacy and hence has a great potential in the preparation of drugs used against oralbacterial diseases.