تحديد العوامل الوراثية المسؤولة عن صفة المقاومة للمضادات الحيوية في جرثومة STAPHYLOCOCCUS SCIURI

Abstract

For investigating the genetic factors responsible for antibiotics resistance for Staph. sciuri transformation experiments were carried out with standard strain Escherichia coli JM83 using DNA plasmid purified from 5 strains of Staph. scriui posses predominant resistance differences between (6-24) antibiotics. The mechanism responsible for antibiotic resistance was tested to produc Extend Spectrum Beta lactamase (ESBLs) after using electrophoresis technique using Polyacrylamide gel with sodium dodocyl sulfate (SDS – PAGE) for BLs extracted from the 5 strains produced it and it revealed (1-3) bands with m.wt. between (20-26) KDa...As regard responsible for It was found that this genes are located on plasmid DNA molecules and these results were supported by electrophoresis of DNA purified from plasmid transformed colonies of laboratory strains of E. coli JM83, a single band of plasmid DNA on agarose gel appeared to have between (1-4) bands according to the types of the strains. On the other hand, one strain couldn't give any band which may indicate the occurrence of gene code for antibiotic resistance may be carried by the chromosome too.