Modification of ultrasound cavitation effect induce changes in the cell redox functional activity by means of chemiluminescence.


The cavitation effect of ultrasound (U S) in vivo situation can be monitored in vitro by the changes in the redox activity of yeast cells using lucigenin dependent chemiluminescence (CL). This technique can be used to detect the cavitational harmful effects of different ultrasound frequencies used in medical diagnosis.CL of yeast cells is produced by excitation of lucigenin on the oxidative free radicals and hydrogen peroxide, in the suspension medium. The changes in the redox activity was monitored at different viscosities of the medium. The results suggest that increase in the viscosity of the suspension medium reduced the cavitational events. This appear clearly as the( % CL) decreases significantly(P<0.05) with decreasing the viscosity of the suspended medium equivalent to greater than(0.3gm/100ml).