DETECTION OF A, A- MUTATIONS OF G6PD GENE ON MOLECULAR LEVEL IN IRAQI POPULATION

Abstract

The study involved the extraction of deoxyribonucleic acid (DNA) from 71 samples of random G6PD patients and 85 samples apparently healthy individuals from different Iraqi populations respectively, which was then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzymes (Nla III and Fok I) to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely A and A-. The results of the current study showed that Iraqis were affected by G6PD deficiency in a percentage 7.2% and showed that the affected cases were attributed to A mutation in 4.2% while 0% was recorded for A- mutation.