Effect of Trigonella Foenum-Graecum L. (Fenugreek) on Liver Enzymes in Ischemia-Reperfusion Injury


ABSTRACT:BACKGROUND:Hepatic ischemia-reperfusion (I/R) injury may occur in a variety of clinical set¬tings and this remains a significant problem. Oxygen free radi¬cals, produced on reperfusion have been shown to play a major role in hepatic I/R injury. Various therapeutic effects have been described for Trigonella. Additionally, it has been presented that Trigonella has protective effect against ischemia reperfusion injury to various organs. Therefore, it seems possible that the administration of Trigonella might protect the liver against the ischemia reperfusion injury.OBJECTIVE:To determine whether Trigonella foenum-graecum L. (Fenugreek) prevents hepatic ischemia-reperfusion injury to the liver.METHODS: Thirty-six rats were divided into three groups as, (Group 1) control group, (Group 2)I/R group and (Group3) Trigonella foenum-graecum L. (Fenugreek) treatment group. All rats underwent hepatic ischemia for 60 min followed by 60 min period of reperfusion. Rats were internal infused with only 0.9% saline solution in group 2. Rats in group 3 received hydro alcoholic - extracted Trigonella foenum-graecum L. (Fenugreek) (500 mg/kg)internal, before isch¬emia and before reperfusion. Blood samples were harvested from the rats, and then the rats were sacrificed. Serum aspartate aminotransfera¬se (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) levels were determined.RESULTS:The levels of liver enzymes in group 3 were significantly lower than those in the group 2.CONCLUSION: Our results suggest that Trigonella foenum-graecum L. (Fenugreek) treatment protects the rat liver against hepatic ischemia-reperfusion injury