Antibacterial Activity of Mentha Piperita and Allium Sativum Against Some of Gram-ve Bacteria


The aqueous extracts of Peppermint (Mentha piperita) and garlic (Allium sativum) with five concentrations (0.1, 0.2, 0.3 , 0.4 and 0.5mg/ml) of each one were tested for antibacterial activities against pathogenic bacteria like: Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa and Proteus vulgaris . The testing was performed by well diffusion method. Both extracts of (peppermint, garlic) showed clear antimicrobial activity against pathogenic bacteria, and this activity was enhanced with the increasing of concentrations belongs to them. The concentration (0.5mg/ml) of both extracts gave highest activity against these bacteria; therefore we screened a mixture of equivalent ratio 1:1 of both extracts in this concentration which has exhibited a significant result (average zone of inhibition 27 mm). Antimicrobial activity of both extracts was compared with that for a number of antibiotics that include: ampicilin, cephalexin, erythromycin, amoxicillin, gentamicin , tetracyclin, chloromphenicol, nalidixic acid and trimethoprim by using antibiogram test.