Spectrophotometric Determination of Cefixime Through Schiff’s Base System Using Vanillin Reagents Inpharmaceutical Preparations


Abstract A simple, rapid, accurate and sensitive spectrophotometric method for the determination of cefixime has been developed. The method is based on the formation of the schiff’s base between the primary amino group present in cefixime and aldehyde group present in the vanillin reagent to produce a yellow colored complex having maximum absorption at 414 nm. Beeˈs law was obeyed over the concentration range of 2-20µg/ml,with molar absorptivity of 8.22x104 l/mol.cm. The present method is considered to be simple because it does not need heating, hydrolysis and solvent extraction steps. The ingredients often formulated with cefixime have been shown not to interfere, and is suitable for the routine determination of cefixime. The proposed method has been successfully applied for the determination of cefixime in pure form and in pharmaceutical preparations(capsules)