The role of Indirect Immunofluorscent test in diagnosis of Candida albicans in Iraq

Abstract

Abstract:Objectives: to study immunodiagnostic of Candida albicans by IFAT, which illustrate the reaction at 1:16 dilution to 1:256 with infected sera.Materials & Methods: One hundred vaginal swabs and blood samples were obtained from non – pregnant women (i.e. marriage women) with vaginal discharge. Blood samples for serum separation, to study antibody titer by using indirect Immunofluorscent test (IFAT). During the period between September 2002 to May 2003. Attending to out patients clinic of Obstetric and Gynecology of Medical city /Baghdad teaching Hospital, Results: Out of the one hundred examined women patients, Candida spp. was isolated from 80 patients. Among the 80 isolated of Candida spp. 51(63.7%) were C. albicans. 41(51.25%) of infected women were positive for specific IgG and 10(12.5%) of them specific for IgM against C. albicans by IFAT.Conclusion: This study was carried out, to apply IFAT test for Candida albicans. Key words: Candida albicans, IFAT, Vaginal discharge.