Spectrophotometric Determination of Salbutamol by Oxidative Coupling Reaction with N, N- Dimethyl para phenylene diamine dihydrochloride in the Presence of Potassium Ferricyanide in Basic Medium


This research involves a simple, rapid and sensitive spectrophotometric method for the determination of Salbutamol . The method based on the oxidative coupling reaction of salbutamol with N, N- Dimethyl para phenylene diamine dihydrochloride( DMPPDAD) using Potassium Ferricyanide as oxidizing agent in alkaline medium to form a water soluble product , that is stable and has a maximum absorption at 643 nm. pH between 10.7 -11.7 . at temperature between 20-35 oC. Beer’s law is obeyed in a concentration range 5 to 25 µg / ml with a molar absorptivity of 5719.27 L.mol-1. cm-1, The product was stable for 40 minutes with relative error was 0 to - 0.36 % and a relative standard deviation of 0.78 to 1.96% ,DL= 0.29 µg / ml, RE=99.69% depending on the concentration of Salbutamol. The proposed method has been successfully applied to the determination of Salbutamol in inhaler solution