Spectrophotometric Determination of chlorpromazine Hydrochloride in Pharmaceutical Preparations by Using Oxidative coupling reaction


Abstract A simple, rapid and sensitive spectrophotometric method for the determination of microgram amounts of chlorpromazine Hydrochloride drug in aqueous solution is described. The method is based on the Oxidative coupling reaction between chlorpromazine hydrochloride and para methoxy aniline in the presence of sodium periodate and Sulfuric acid to form an intense red -Violet colored product with maximum absorption at 546 nm. Beer’s law is obeyed over the concentration range of( 0.5 – 11.2) ppm with molar absorptivity of 6.398x104 l.mol-1.cm-1 and Sandell`s sensitivity of 0.005 μg.cm-2 . The method does not resort to temperature control or to solvent extraction. The optimum conditions for all colour development are described and the proposed method has been successfully applied for the determination of chlorpromazine hydrochloride in bulk drug and pharmaceutical Preparations(Largactil drug). The common excipients and additives did not interfere in this method