PRODUCTION AND PURIFICATION OF EXTRA CELLULAR LIPASE FROM PSEUDOMONAS CEPACIA AND STUDY THE PARTIALCHARACTERIZATION .

Abstract

Twelve isolates of Pseudomonas were obtained from thirty five(34%)from different species soil ,plants and river s water in al Muqdadia fields.Ability of lipase production by these isolates was screened PseudomonasP5 was highest lipase producer identified as Pseudomonas cepacia .Lipase production conditions were studied .the highest production oflipase was observed when mineral medium containing 5% linseed oilinculcated with bacterial cells e ,ph 7.5 and incubated at40 c in shakingincubator120 r.p.m for 18 hr.Lipase was purified from bacterial cells by two steps includingprecipitation with ammonium sulfate and Ion exchange chromatography byDEAE- Sephadex-A25, fold of purification was 35 Uml with 36.5%enzyme recovery .The effect of some metal ions on lipase activity was studied .The Ca+2increased lipase activity to 150%.The effect of specific substances on enzyme activity was investigated,The result showed that Tween80 raised lipase activity to 145%.