Spectrophotometric Determination of Propranolol Hydrochloride and Application to Pharmaceutical Preparations

Abstract

A spectroscopic method has been used in this study for determining the drug compound (Propranolol Hydrochloride) as a pure substance and in pharmaceutical preparations using UV-Vis. spectroscopy. The procedure included reduction of Fe (III) ions to Fe (II) ions which reacted with potassium, hexa ferriccyanide, to form a bluish-green precipitate soluble in acidic solution. The maximum absorption has been measured at wavelength (726)nm. The reaction conditions have been studied including sequence additions, concentration and volume of reactants, acidity, temperature and time of reaction. The optimum conditions of reaction have been fixed and two methods have been used for determining Propranolol hydrochloride (PPH), the first, is the direct standard calibration curve, and the second is standard additions curve. Both methods showed linearity range between (0.25-7.0)µg.ml-1, detection limit (0.084)µg.ml-1, correlation coefficient (r) = 0.9998 and the absorption coefficient molarities for the complex formed (Ɛ) = 2.9 ×104 L.mol-1.cm-1, and sandel sensitivity = (0.0007)µg.cm2, recovery percentage value (%Rec.) = 99.932, the relative standard deviation (%RSD) = 0.5, which means that there are matching between both two methods in determination clearly.