The Role of Some B Vitamins in Methionine-Induced Hyperhomocysteinemia in Male Rabbits


ackground: Hyperhomocysteinemia has been associated with an increased risk ofatherosclerosis and other cardiovascular diseases. Vitamin B6, vitamin B12, and folicacid are essential components in the metabolism of homocysteine.Objective: This study aimed to assess the effects of vitamin B6, vitamin B12, folic acid, andthe combination of them in the prevention of hyperhomocysteinemia.Methods: Thirty six male local rabbits were used in the study. Methionine was given torabbits to induce a model of hyperhomocysteinemia. Rabbits were divided randomly into 6groups (6 rabbits in each group) as the following: control group: they were maintained onstandard chow only; methionine only group: they were maintained on methionine only; B6group: they were maintained on methionine and vitamin B6; B12 group: they weremaintained on methionine and vitamin B12; folic acid group: they were maintained onmethionine and folic acid; multivitamins group: they were maintained on methionine,vitamin B6, vitamin B12, and folic acid.Results: Multivitamins group showed no significant differences (P>0.05) in homo-cysteinelevels at day 30 in comparison with day 0 and there are no significant differences (P>0.05) inhomocysteine levels at day 30 between multivitamins group and control group. At day 30 andday 60, serum homocysteine levels in multivitamins group were significantly (P<0.05) lowerthan serum homocysteine levels in folic acid group.Conclusion: Multivitamin combination composed of vitamin B6, vitamin B12, and folic acidreduces the hyperhomocysteinemic effect of methionine and it is better than folic acid alone,while folic acid is better than vitamin B6 or vitamin B12 when they used alone.