Immunofluorescence and the Spectrum of Glomerular Diseases in Iraq (Single Center Study)


objective: Immunofluorescent microscopy is an important tool for the diagnosis of glomerular diseases. In this study, we focused on using IF technique together with light microscopy and clinical features in the diagnosis of different types of glomerulonephritis. Spectrum of glomerular disease in Iraq is to be studied and compared with other studies in Iraq and other countries.Methods: A total of 58 kidney biopsies were taken for routine LM, while IF technique was done in 56 of them. The study started from 1st of June 2010 to the 1st of June 2012.Results: Focal segmental glomerulosclerosis (17/58=29.3%) topped the list followed by minimal change disease(12/58=20.7%).Immune deposition was observed in (21/56=37.5%) cases and the predominant deposit was immunoglobulin G(20/56=35.7%).The pattern of deposition was granular in most of the cases either in the glomerular basement membrane and/or in the mesangium except one case where immunoflourecent microscopy showed dominant positive staining (3+) for complement factor 1q in the glomerular mesangium and slightly positive staining for complement factor 3 in the same mesangeal areas.(Tow/58=3.4%) cases fulfilled the clinical, serological and histopathological criteria of lupus nephritis .Conclusion: To obtain a correct diagnosis of glomerulonephritis, we require immunofourescent in parallel with light microscopic examination of renal biopsies and correlation with clinical features, biochemical and serological markers. Clinically, the majority of patients presented as nephrotic syndrome (44/58=75.9%).Key words: Immunofluorescence,glomerular disease.