Detection of The Antibacterial Activity of Bacteriocin from Local Isolates of Pseudomonas fluorescens Against Gram Positive Bacteria


AbstractThe antibacterial activity of local isolates of Pseudomonas fluorescens (P1and P2)were tested against some pathogenic bacteria, Staphylococcus aureus, S. epidermidis,Streptococcus faecalis, Streptococcus faecium isolated from stool urine and wounds bymodified agar block method at 370C for 24 hr. The isolates of P. fluorescens were positive asproducer of bacteriocin with a wide inhibition range on growth of gram positive pathogenicbacteria. P1 inhibited the bacterial growth of S. aureus isolates with a range of inhibition zone(12-20) mm. while P2 inhibited the bacterial growth with a range of inhibition zone was (13-22) mm .The range of inhibition zones of S.epidermidis by P1 were (10-18) mm. while therange by P2 was (9-20) mm. The isolate P1 inhibited the growth of all the tested isolates ofS. faecalis with a range of inhibition zone between (12-19) mm. The range of inhibition zonesof S. faecalis by P2 were (10-18) mm. The bacterial growth of S. faecium isolates wereinhibited by P1 with a range of (15-19) mm. and (16-19) mm. with P2. Agar block methodwas suitable and easy for screening of bacteriocin production from this bacterium.