Measuring the humoral immune response to various vaccine programs against Newcastle disease in broiler using hemagglutination test and ELISA test

Abstract

The present study carried out to assess different vaccination programs against Newcastle disease to know the best program which provides bold protection against Newcastle disease. The present study designed to contain four groups of broiler chicks (type Hubbard), and vaccination programs done as following:T1 vaccinated at day old by spraying method with live vaccine type (B1), then vaccinated at 10, 20, and 30 days old with live vaccine type (Lasota) by drinking water method. T2 vaccinated at day old by spraying method with live vaccine type (B1), then vaccinated with killed vaccine at five days old by injecting subcutaneously at neck region.T3 vaccinated at day old by spraying with live vaccine type (B1), then vaccinated at 7, and 18 days old with live vaccine type (Lasota) by drinking water method. T4vaccinated at 7, 18, and 35 days old with live vaccine type (Lasota) by drinking water method. Maternal immunity assessed at 2 days old then Immune response assessed in 14, 24, 34, and 44 days old by heamaglutination inhibition test (HI) test and enzyme linked immunosorbent assay (ELISA), and daily body weight recorded. The results of immune response tests revealed there is non-significance between experimental groups at 1, 14 days old (P≤0.05), while there is highly significance in antibody level between T2 and other groups at 24 days old (P≤0.05). In the 34th days old there is significance between T2 and T3 only while there is insignificance between other groups (P≤0.05). In 44th days old there is high level of antibody in T2 which is significant in compare with other experimental groups (P≤0.05).The present study provided the evidence of efficacy of the program that used killed vaccine which preceded with live vaccine by spraying method and followed by live vaccine by drinking water method in compare with other famous usable programs, and this efficacy represented in the levels of immune response and body weight, the present study also showed that increasing of vaccination numbers considered stress factor as well as it provide solid immunity against Newcastle disease.