A Biocompatibility Study of a New Root Canal Sealer


Aims: The purpose of this study was to evaluate the reaction of the subcutaneous connective tissue to a new sealer composed of ethanolic extract of Propolis (EEP) and zinc oxide powder. Materials and Methods: Twenty five adult rats (Rattus novergicus, Albinos wistar) weighting between 200 and 250 grams, provided by central animal house of Mosul University of the Veterinary College were used. Materials were autoclaved polyethylene tubes, 10mm long with a single lumen and an inner diameter of 1 mm, ensuring that air was not entrapped. The procedure was preformed in the subcutaneous tissue of the rat in the dorsum of the animal following the sagittal line between the frontal legs, was submitted to trichotomy for exposure of the skin, followed by a sepsis with a gauze soaked with 70% alcohol. Results: No rats died during the experimental study. The surviving skin appeared normal in this texture and bled when cut, compared to negative control. The connective tissue responded along the lateral wall outside the polyethylene tubes of all specimens also served as a negative control. A moderate in-flammatory reaction was observed in the second day when new sealer was used. The tissue reaction showed a focal neutrophilic inflammatory infilterates. On the 7th day a mild to absent inflammatory reaction were observed. The histological features on the 14th, 30th and 60th days showed similar fea-tures. Conclusions: The results obtained in the present study allowed the conclusion that the new root canal sealer composed of zinc –oxide (powder) and EEP (liquid) presented good biocompatibility.